CHARACTERIZATION OF RIFAMYCIN OXIDASE IMMOBILIZED ON ALGINATE GEL

Authors
BANERJEE UC
Citation
Uc. Banerjee, CHARACTERIZATION OF RIFAMYCIN OXIDASE IMMOBILIZED ON ALGINATE GEL, Biomaterials, artificial cells, and immobilization biotechnology, 21(5), 1993, pp. 675-683
Citations number
16
Categorie Soggetti
Engineering, Biomedical","Material Science
Journal title
Biomaterials, artificial cells, and immobilization biotechnology → ACNP
ISSN journal
10557172
Volume
21
Issue
5
Year of publication
1993
Pages
675 - 683
Database
ISI
SICI code
1055-7172(1993)21:5<675:COROIO>2.0.ZU;2-6
Abstract
Rifamycin oxidase of Curvularia luntata was immobilized on alginate ge l. The pH and temperature optima of the immobilized enzyme preparation were 6.5 and 50-degrees-C, respectively. Transformation reaction was carried out with the immobilized enzyme preparation. It took 8 h for t he complete transformation of rifamycin B (10 g/L) to rifamycin S. The immobilized enzyme preparation was found to be mechanically weak even in the presence of CaCl2 in the reaction mixture. Reusability studies showed that the catalyst can not be repeatedly used very effectively.