ISOENZYMES OF LACTATE-DEHYDROGENASE IN PU REBRED AND CROSSBRED EWES AND RAMS

In farm animals the questions of clinical and mainly heritable health receive increased attention. In this work the activity of the polymorp hous system of lactate dehydrogenase was observed in some pure- and cr ossbred ewes and rams under field conditions. The isoenzyme of lactate dehydrogenase were separated on 5.5 % polyacrylamid gel as reported D ietz, Lubrano (1967). Age- and sex-related determinations of the isoen zyme spectrum of lactate dehydrogenase revealed increased LD3 activity in yearling ewes, and increased LD4 and LD5 activity in yearling rams (Tab. I). Significant differences of LD4 isoenzyme activity (P < 0.05 ) were observed between the Stavropol Merino and the Stavropol Merino x Merino x Corriedale crossbreds (Tabs II and III). In the blood plasm a of Stavropol Merino rams the following quantities of LD isoenzymes w ere determined: LD1 = 48.0 +/- 10.6 %, LD2 = 12.1 +/- 5.2 %, LD3 = 25. 9 +/- 7.02 %, LD4 = 4.0 +/- 2.2 % and LD5 = 10.0 +/- 3.5 %. In the act ivity of some isoenzymes breed differences were recorded in 2-5-year-o ld ewes (Tabs IV and V). For LD1, significant differences (P < 0.01) w ere found between the Merino and Ascan Merino x Merino sheep breeds as well as between the Merino x Corriedale sheep and the crossbreds of M erino with a certain share of Lincoln. Differences in LD1 activity bet ween Merino and Merino x Corriedale sheep as well as between the Ascan Merino and Merino crossbreds with a certain share of Lincoln were sig nificant at the level of P < 0.02. For LD2, no significant differences were stated in the pure- and crossbred animals under examination. LD3 values in the Ascan Merino x Merino and Merino x Corriedale sheep sig nificantly differed from those observed in the crossbreds of Merino wi th a certain genetic share of Lincoln (P < 0.01). Different LD4 and LD 5 activities were determined in die Merino and Ascan Merino x Merino s heep (P < 0.02) as well as in the Ascan Merino x Merino and the crossb reds of Merino with a certain share of Lincoln (P < 0.01). In the Meri no breed the following LD is isoenzyme activities were recorded: LD1 = 48.5 +/- 9.5, LD2 = 10.1 +/- 3.3 %, LD3 = 28.1 +/- 6.5 %, LD4 = 5.8 /- 3.5 % and LD5 = 7.5 +/- 6.1 %.