REAL-TIME KINETIC-ANALYSIS APPLIED TO THE PRODUCTION OF BISPECIFIC MONOCLONAL-ANTIBODIES FOR RADIOIMMUNODETECTION OF CANCER

An automated biosensor system designed for measuring molecular interac tions in real-time and without labelling of the reactants has been use d to evaluate the association/dissociation rate and affinity constants of bivalent monoclonal antibodies and a monovalent bispecific monoclo nal antibody. Observed differences in affinity between parental and bi specific antibody produced were related to the association rate consta nts, since the dissociation rate constants were in the same range. Val ues were also closely related to radioimmunochemical data. These resul ts indicate that the biosensor system, besides presenting several adva ntages for characterizing antigen-antibody interaction, is valuable fo r selecting monoclonal antibodies with properties which might be usefu l in the development of bispecific monoclonal antibodies.