Ma. Miller et al., A METHOD FOR SIMULTANEOUS DETECTION OF MULTIPLE MESSENGER-RNAS USING DIGOXIGENIN AND RADIOISOTOPIC CRNA PROBES, The Journal of histochemistry and cytochemistry, 41(12), 1993, pp. 1741-1750
We present a simple, reliable method for simultaneous detection of two
distinct mRNAs within the same tissue sample by double in situ hybrid
ization histochemistry. Sections are hybridized with a cocktail of rad
iolabeled and digoxigenin-labeled cRNA probes. The digoxigenin-labeled
probe is detected with an alkaline phosphatase (AP)-dependent chromog
en reaction and then the radiolabeled probe is detected by conventiona
l autoradiography. The sensitivities of the two detection methods are
comparable and demonstrate the feasibility of using double in situ hyb
ridization histochemistry to investigate the co-localization and co-re
gulation of mRNAs that are expressed at relatively low levels.