IMMUNOELECTRON MICROSCOPY OF PARAINFLUENZA VIRION GLYCOPROTEINS WITH POLYCLONAL AND MONOCLONAL-ANTIBODIES - MOVEMENT OF GLYCOPROTEIN WITH MONOCLONAL-ANTIBODY

Two glycoproteins (HN and F) of parainfluenza virus were immunogold-la beled with polyclonal and monoclonal antibodies, respectively, and the ir labeling patterns were compared. Both glycoproteins HN and F were e fficiently and homogeneously labeled with polyclonal antibodies, where as they were labeled much less and heterogeneously with monoclonal ant ibodies. When either protein was initially labeled with monoclonal ant ibody, and then the other one, with polyclonal antibody, immunolabels of two glycoproteins were almost completely segregated. Although this segregation deformed virion morphology, it supported the concept of mo noclonal antibody-mediated movements of glycoproteins.