ELIMINATION OF SPECTRAL INTERFERENCES IN THE REACTION OF 2-THIOBARBITURIC ACID WITH MALONALDEHYDE, 2-FURFURALDEHYDE AND 5-HYDROXYMETHYL-2-FURFURALDEHYDE BY PARTIAL LEAST-SQUARES MULTIVARIATE CALIBRATION (PLS)

The spectral resolution of ternary mixtures of malonaldehyde (MLD), 2- furfuraldehyde (FUR) and 5-hydroxymethyl-2-furfuraldehyde (HMF) in the presence of glyoxal and biliverdine is achieved by partial least squa res multivariate calibration (PLS). The spectrophotometric method is b ased on the reaction of these substances with 2-thiobarbituric acid (T BA). A calibration set of standard samples has been statistically desi gned in the presence of adequate amounts of both interferent compounds in several degrees of concentration. The possibility of a spectrophot ometric determination of mixtures of MLD, FUR and HMF in the presence of glyoxal and biliverdine is demonstrated. A comparative study of the results found by application of PLS-1 and PLS-2 methods is presented.