Bv. Joypaul et al., ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR P53 IN GASTROINTESTINAL MALIGNANCY - COMPARISON WITH IMMUNOHISTOCHEMISTRY, Histopathology, 23(5), 1993, pp. 465-470
Mutations in the p53 nuclear oncogene occur frequently in a wide spect
rum of human malignancies and the mutant protein may prove to be a use
ful diagnostic or prognostic marker. It can be detected in fixed tissu
es by immunohistochemistry, but the type of fixative and conditions of
fixation used can introduce variability. For routine clinical use, a
method of analysis which is more easily standardized would, therefore,
be of benefit. A two-site enzyme-linked immunosorbent assay (ELISA) w
as used to measure the level of p53 protein in soluble extracts from 2
0 gastrointestinal cancers (11 colonic, nine gastric). Immunohistochem
istry was also performed on the paraffin-embedded sections of these tu
mours and the results of the two assays were compared. ELISA detected
p53 at Various levers in 10 cases, all of which were also positive by
immunohistochemistry. Of the other 10, eight were immunohistochemicall
y negative but two were positive. When the immunohistochemically posit
ive specimens were ranked by scoring the degree of staining, there was
a highly significant correlation with the quantitative ELISA results.
Our study shows that the ELISA is sensitive and highly specific. It o
ffers an alternative and simple method of assessing the p53 status in
human tissues.