EXPRESSION AND SECRETION OF WHEAT ALPHA-AMYLASE IN KLUYVEROMYCES-LACTIS

Authors
RUSSELL C JARVIS A YU PL MAWSON J
Citation
C. Russell et al., EXPRESSION AND SECRETION OF WHEAT ALPHA-AMYLASE IN KLUYVEROMYCES-LACTIS, Applied microbiology and biotechnology, 40(2-3), 1993, pp. 306-308
Citations number
16
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Applied microbiology and biotechnology → ACNP
ISSN journal
01757598
Volume
40
Issue
2-3
Year of publication
1993
Pages
306 - 308
Database
ISI
SICI code
0175-7598(1993)40:2-3<306:EASOWA>2.0.ZU;2-0
Abstract
The plasmid pCR1 has been constructed to express a wheat alpha-amylase enzyme in Kluyveromyces lactis strains. The construct is based on the vector pCXJ-kan1, which has been derived from pKD1, a native plasmid of K. lactis var. drosophilarum containing the essential regions for p lasmid replication and stability. Construct pCR1 produces an alpha-amy lase by DNA isolated from a wheat cDNA clone and is controlled by a Sa ccharomyces cerevisiae PGK promoter.