CLONING AND SEQUENCING OF THE URA3 LOCUS OF THE METHYLOTROPHIC YEAST HANSENULA-POLYMORPHA AND ITS USE FOR THE GENERATION OF A DELETION BY GENE REPLACEMENT

Authors
MERCKELBACH A GODECKE S JANOWICZ ZA HOLLENBERG CP
Citation
A. Merckelbach et al., CLONING AND SEQUENCING OF THE URA3 LOCUS OF THE METHYLOTROPHIC YEAST HANSENULA-POLYMORPHA AND ITS USE FOR THE GENERATION OF A DELETION BY GENE REPLACEMENT, Applied microbiology and biotechnology, 40(2-3), 1993, pp. 361-364
Citations number
26
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Applied microbiology and biotechnology → ACNP
ISSN journal
01757598
Volume
40
Issue
2-3
Year of publication
1993
Pages
361 - 364
Database
ISI
SICI code
0175-7598(1993)40:2-3<361:CASOTU>2.0.ZU;2-6
Abstract
The ura3 gene of Hansenula polymorpha was cloned, sequenced and used t o generate a ura3 mutant from the wild-type strain of this yeast via i ntegrative mutagenesis. The Tn5 neomycin-resistance marker (neo) under control of the ADH1 promoter from Saccharomyces cerevisiae served as a transformation marker. The results show that gene replacement can be achieved in H. polymorpha, a yeast with a high level of non-homologou s integration.