W. Sand et al., CONTROLLED MICROBIOLOGICAL IN-SITU SLOPE LEACHING OF A SULPHIDIC ORE, Applied microbiology and biotechnology, 40(2-3), 1993, pp. 421-426
A mixed culture of Thiobacillus ferrooxidans, T. thiooxidans, and Lept
ospirillum ferrooxidans was used for inoculation of a sulphidic ore bo
dy for a bacterial in-situ stope-leaching experiment in the Ilba mine
in Romania. The ore body was inoculated with 10(7) cells/g ore. Measur
ements at six main sites of the ore body indicated that microbial leac
hing was started by the inoculation. After about 8 weeks, sufficient m
icrobial activity was measurable only in the upper third of the ore bo
dy. Due to the angle of incidence of the ore (75 degrees), the leach l
iquor percolated only through the upper part leaving two-thirds humidi
fied unsatisfactorily. The leach results, metal mobilization, indicate
d that by inoculation with the indigenous microorganisms efficient lea
ching was achieved. Metal output after 18 months of operation amounted
to 10% of Cu and 78% of Zn. In the winter months energy for aeration
and circulation was not available and this was reflected by reduced va
lues for microbial activity, temperature, and daily metal output. The
biological metal mobilisation after 18 months of operation was as acti
ve as at the beginning. Cu was mobilised predominantly by microbial le
aching whereas Zn was leached mainly by chemical reactions. Both mecha
nisms contributed equally to iron output.