EVALUATION OF THE BATCH KINETICS OF THE AMINO-ACID-METABOLISM OF A MOUSE HYBRIDOMA AND HUMAN LYMPHOBLASTOID CELL-LINE USING A PRECOLUMN FDNDEA DERIVITISATION, HPLC TECHNIQUE

The analysis of free amino acids in mammalian cell culture media can g ive valuable information on the metabolism of particular commercially valuable cell lines. Analysis of spent culture media indicates potenti al limiting nutrients. However, analysis over the whole culture period enables a kinetic approach to allow understanding of amino acid uptak e and potential strategies to improve media design. This communication details the use of a less commonly used HPLC protocol, indicating var ious advantages and disadvantages. Further, the batch kinetics of amin o acid metabolism of two cell lines are discussed.