GEL-ENTRAPPED PENICILLIN-G ACYLASE OPTIMIZED BY AN ENZYME THERMISTOR

Authors
WELWARDOVA A GEMEINER P MICHALKOVA E WELWARD L JAKUBOVA A
Citation
A. Welwardova et al., GEL-ENTRAPPED PENICILLIN-G ACYLASE OPTIMIZED BY AN ENZYME THERMISTOR, Biotechnology techniques, 7(11), 1993, pp. 809-814
Citations number
10
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
7
Issue
11
Year of publication
1993
Pages
809 - 814
Database
ISI
SICI code
0951-208X(1993)7:11<809:GPAOBA>2.0.ZU;2-X
Abstract
Direct activity determination by a flow-through microcalorimetry in th e enzyme thermistor system was employed for a fast comparison of (poly )acrylamide gel-entrapped penicillin G acylase preparations. Compositi on of the pre-polymerization cocktail and both the storage and operati onal stabilities of optimal gel-entrapped enzyme preparations isolated from the Escherichia coli industrial strain were optimized by this me thod. The validity of the results was corroborated by spectrophotometr ic measurements.