M. Longeri et al., BOLA CLASS-I POLYMORPHISM AND IN-VITRO IMMUNE-RESPONSE TO M-BOVIS-ANTIGENS, Journal of animal breeding and genetics, 110(5), 1993, pp. 335-345
From a sample of 119 Friesian calves, serologically typed for BoLA cla
ss I, 47 subjects were chosen expressing 9 different MHC types (A6, A6
.9, A10, All, A14, A15, A30, W16, M103) with the same age and reared i
n the same farm conditions. The animals were s.c. injected with a wate
r in oil suspension of killed M. bovis and the treatment was repeated
two days later. Before the treatment and 21 days later, calves were bl
ed and on PBM peripheral blood mononuclear leucocytes) were performed
the following tests: 1. Lymphocyte Stimulation with bovine and avian P
PDs (Purified protein derivative of Mycobacterium bovis and Mycobacter
ium, respectively). 2. Phagocytic activity towards M. bovis. 3. Class
II molecules expression on cell surface. 4. Percentage of leucocyte po
pulations and subpopulations. In the in vitro Lymphocyte Stimulation t
est, all the animals and classes were responders. Animals bearing A10
BoLA class I presented c.p.m. (counts per minute) and index values hig
her than the other cattle; these values were significantly positively
related both to bovine and avian PPDs (P < .01). By variance analysis
A14 BoLA type showed a slight positive significant correlation with mo
re efficient phagocytic activity. BoLA class I type did not seem to si
gnificantly affect percentage of class II positive cells and leucocyte
percentages on PBM.