HYDROLYSIS OF BEEF TALLOW BY IMMOBILIZED LIPASE IN A BIPHASIC ORGANIC-AQUEOUS SYSTEM

Lipase from Candida cylindracea was immobilized on chitosan beads by c ovalent binding methods, and its application to hydrolysis of beef tal low was investigated in a biphasic organic-aqueous system. The additio n of isooctane, found to be the best among the organic solvents tested , to the reaction system stimulated the hydrolysis of beef tallow by i mmobilized lipase strongly. The proportion of isooctane and aqueous bu ffer in the reaction mixture was chosen at the ratio of 1 : 4. Under t his condition, the reaction rate was greatly enhanced compared to that in the organic-solvent-free system, and the extent of hydrolysis reac hed to about 90%.