EXPRESSION OF C-MYC MESSENGER-RNA AND PROTEIN IN HUMAN ENDOMETRIAL CARCINOMA - SIMULTANEOUS STUDY OF IN-SITU HYBRIDIZATION AND IMMUNOHISTOCHEMISTRY

We examined the c-myc gene at both mRNA and protein levels in tumors o f 10 endometrial carcinomas by simultaneous in situ hybridization and immunohistochemistry. Expression of c-myc mRNA was observed in all cas es by in situ hybridization. Immunohistochemically, c-myc protein expr ession was also observed in all cases and immunoreactivity was recogni zed as nuclear staining. The site of c-myc protein expression was most ly consistent with that of c-myc mRNA expression. While there has been no clear correlation between the expression of c-myc mRNA and/or prot ein and prognostic parameters so far, this approach will contribute to an understanding of oncogene expression and its biological significan ce in human gynecological malignancies.