A 5.5-MB HIGH-RESOLUTION INTEGRATED MAP OF DISTAL 11Q13

The distal part of 11q13, which contains several genes relevant to hum an diseases, has been poorly mapped as part of genome-wide mapping eff orts. In the prospect of drawing a fine-scale integrated map of the ar ea containing KRN1 and OMP, we have established a framework of markers by hybridization to DNA of somatic cell hybrids and by fluorescence i n situ hybridization (FISH) on metaphase chromosomes. The probes studi ed were used to isolate 27 YACs and 16 cosmids that could be organized in three contigs covering approximately 6 Mb. These contigs were sepa rated by two gaps that are likely to contain sequences underrepresente d in YAC libraries. They were then integrated based on long-range rest riction mapping and DNA-fiber FISH into a high-resolution physical map , which covers a 5.5-Mb region and includes 36 anonymous markers and 1 0 genes. This map will be used to search for genes within the 2/3 of t his region where none have been localized as yet. It will also lay the ground for the characterization of an amplicon surrounding GARP in br east cancer and for the search of disease genes within this region. (C ) 1997 Academic Press.