PHYSICAL MAPPING OF THE HUMAN CONNEXIN-40 (GJA5), FLAVIN-CONTAINING MONOOXYGENASE-5, AND NATRIURETIC PEPTIDE RECEPTOR-A GENES ON 1Q21

The human connexin 40 gene (Cx40; HGMW-approved symbol GJA5), a gap ju nction protein, was mapped previously to 1pter-q12 by analysis of soma tic cell hybrids. In the development of a yeast artificial chromosome (YAC) contig at 1q21, a YAC end clone contained the entire 5'-untransl ated region and 42 bases of coding region of the Cx40 gene. Using a se quence-tagged site (STS) developed from this sequence as well as ampli mers for the natriuretic peptide receptor A (NPR1) and flavin-containi ng monooxygenase 5 (FMO5) genes, the order NPR1-FMO5-Cx40 was establis hed, with Cx40 being the most telomeric. Single-color FISH using PAC c lones containing the Cx40 and NPR1 STSs localized these genes to 1q21. 1. (C) 1997 Academic Press.