IDENTIFICATION OF MONOCLONAL-ANTIBODIES SPECIFIC FOR BOVINE LEUKOCYTECOMMON ANTIGEN (CD45) TOGETHER WITH A NOVEL BROADLY EXPRESSED LEUKOCYTE DIFFERENTIATION ANTIGEN, BOWC11

Ten mAbs that comprised temporary clusters TC5 and TC7, plus an additi onal mAb, CC171, were compared. All mAbs reacted broadly and stained a ntigen expressed on thymocytes as well as T and B cells in peripheral blood. The five mAbs CC1, CC171, 2E8, TD14 and TD15 precipitated molec ules of 180, 205 and 220 kDa, and we conclude that they recognize the bovine leukocyte common antigen and are BoCD45 mAb. The three mAbs IL- A117, IC1O and BAGB27A precipitated molecules of 86 and 63 kDa that we re broadly expressed. It is proposed that these three mAbs should be r egarded as a cluster (named BoWC11 within the Second Workshop) that is distinct from BoCD45.