COMPARISON OF WORKSHOP CD45R MONOCLONAL-ANTIBODIES WITH OVCD45R MONOCLONAL-ANTIBODIES IN SHEEP

The reactivity of the antibovine monoclonal antibodies (mAbs) comprisi ng temporary cluster TC1 was compared with that of two OvCD45R mAbs on sheep cells. Three of the mAbs-CC31, CC99 and CC103-did not cross-rea ct with sheep cells. All the workshop mAbs precipitated two molecules of apparent molecular weight (MW) 200 kDa and 220 kDa while the antish eep CD45R mAb 20-96 precipitated a single band of 220 kDa. Cell surfac e expression was examined by single colour FACS (fluorescence activate d cell sorting) analysis of efferent and afferent lymph cells and peri pheral blood lymphocytes and the distribution of the antigens on CD4+, CD8+ and T19+ (WC1) and B cells was determined by two colour fluoresc ence staining. By cellular distribution and immunohistology the TCl mA bs could be divided into four distinct groups which differed from a fi fth group comprising the two OvCD45R antibodies.