IDENTIFICATION OF MONOCLONAL-ANTIBODIES SPECIFIC FOR THE GAMMA DELTA TCR/

Twelve monoclonal antibodies (mAbs) formed temporary cluster TC36. mAb CACTB6A was included as a standard antibody that reacted with the gam ma/delta T cell receptor (TCR). Eight of the mAbs, CACTB6A, CACT16A, C ACT17A, CACTB12A, CACTB44A, CACT71A, CACT18A and CACT61A, immunoprecip itated a 47 kDa molecule indicating that they all recognize the gamma/ delta TCR. Flow cytometric studies separated these mAbs into three gro ups that distinguish three different gamma/delta TCR. mAb BAQ72A, whic h fell within TC36, immunoprecipitated a 215 kDa molecule with a simil ar tissue and cellular distribution to BoWC1 mAb. mAbs CACT26A, CACT63 A and CACT77A reacted similarly to each other by flow cytometry and im munohistology, but were distinct from the other mAbs in TC36. These th ree mAbs may recognize an activation molecule and form a distinct nove l mAb cluster.