ANALYSIS OF THE MONOCLONAL-ANTIBODIES COMPRISING WC6

The WC6 antibovine monoclonal antibodies (mAbs) CC98, IL-A114 and IL-A 53 were investigated for reactivity in sheep by (fluorescence activate d cell sorting) FACS analysis, immunoprecipitation and immunohistology . The mAbs behave identically by all criteria although IL-A114 reacts more weakly than the other mAbs. This probably reflects limited cross- species reactivity. The mAbs stain <30% of lymphocytes from blood, eff erent and afferent lymph and the majority of afferent lymph dendritic cells. They also weakly stain granulocytes. They precipitate molecules of apparent molecular weight 220 kD and 180-190 kD. Sequential immuno precipitation shows that CC98 antigen is not related to CD45. Immunohi stology indicates staining of B cell areas and macrophages in Peyer's patch and lamina propria. The data show that these monoclonal antibodi es react with a molecule distinct from OvCD45R.