NONENZYMATIC VERSUS ENZYMATIC HEPATOCYTE ISOLATION FROM PIG LIVERS FOR LARGER SCALE INVESTIGATIONS OF LIVER-CELL PERFUSION SYSTEMS

Authors
GERLACH JC BROMBACHER J COURTNEY JM NEUHAUS P
Citation
Jc. Gerlach et al., NONENZYMATIC VERSUS ENZYMATIC HEPATOCYTE ISOLATION FROM PIG LIVERS FOR LARGER SCALE INVESTIGATIONS OF LIVER-CELL PERFUSION SYSTEMS, International journal of artificial organs, 16(9), 1993, pp. 677-681
Citations number
26
Categorie Soggetti
Engineering, Biomedical
Journal title
International journal of artificial organs → ACNP
ISSN journal
03913988
Volume
16
Issue
9
Year of publication
1993
Pages
677 - 681
Database
ISI
SICI code
0391-3988(1993)16:9<677:NVEHIF>2.0.ZU;2-M
Abstract
A comparison of nonenzymatic and enzymatic hepatocyte isolation was pe rformed on pig livers. The collagenase perfusion showed superior resul ts: mean viability 72 +/- 10% versus a maximum viability of 21% using EDTA-perfusion. A five-step collagenase perfusion technique, developed for pig livers enables larger scale investigations, in order to devel op methods for hepatocyte cultures in therapeutical liver cell perfusi on systems.