Gj. Vandam et al., SCHISTOSOMA-MANSONI CIRCULATING ANODIC ANTIGEN BUT NOT CIRCULATING CATHODIC ANTIGEN INTERACTS WITH COMPLEMENT COMPONENT C1Q, European Journal of Immunology, 23(11), 1993, pp. 2807-2812
Adult schistosome parasites, living in the blood vessels of their mamm
alian hosts, protect themselves against immune damage in a variety of
ways. In addition to the tegument, the intestinal epithelium of the bl
ood-feeding worms is permanently exposed to both the innate and the ac
quired immune system. In this study, we investigated whether the Schis
tosoma gut-associated antigens CAA and CCA (circulating anodic antigen
and circulating cathodic antigen, respectively), which are excreted i
n relatively large quantities into the host's circulation, might play
a role in evading complement attack. Of several complement components
tested, only purified C1q showed significant binding to CAA, a negativ
ely charged highly glycosylated glycoprotein. CCA, also highly glycosy
lated, but neutral or slightly positively charged, did not bind to C1q
. CAA bound only to the collagen-like stalks of C1q and not to the glo
bular heads. No detectable interaction of CAA with precursor human C1
was found and CAA did not induce activation of C1 in whore human serum
as assessed by consumption of hemolytic C4 activity. Also CAA could n
ot induce activation of precursor C1 in vitro. These results suggest t
hat CAA behaves like a receptor for C1q, and might be involved in prot
ecting the vulnerable schistosome gut against complement-mediated atta
ch.