APPLICATION OF THE TIME-RESOLVED IMMUNOFLUOROMETRIC ASSAY TO THE STUDY OF C3 COMPLEMENT COMPONENT GLYCATION IN-VITRO AND IN-VIVO

Authors
GAILLARD O MEILLET D BORDASFONFREDE M KHALIL L GALLI J DELATTRE J
Citation
O. Gaillard et al., APPLICATION OF THE TIME-RESOLVED IMMUNOFLUOROMETRIC ASSAY TO THE STUDY OF C3 COMPLEMENT COMPONENT GLYCATION IN-VITRO AND IN-VIVO, European journal of clinical chemistry and clinical biochemistry, 31(11), 1993, pp. 749-752
Citations number
16
Categorie Soggetti
Biology,"Chemistry Medicinal
Journal title
European journal of clinical chemistry and clinical biochemistry → ACNP
ISSN journal
09394974
Volume
31
Issue
11
Year of publication
1993
Pages
749 - 752
Database
ISI
SICI code
0939-4974(1993)31:11<749:AOTTIA>2.0.ZU;2-W
Abstract
A time-resolved immunofluorometric assay (TR-IFMA) was used for the me asurement of glycated C3. The very high sensitivity of this technique allowed the direct measurement of glycated and non-glycated proteins ( especially C3) in chromatography eluates. C3 glycation in vitro after incubation with 20 mmol/l glucose was always less than 3.5% by day 5. As determined with the TR-IFMA, the means +/- standard deviations of g lycated C3 were 0.20% +/- 0.04 for non-diabetic subjects and 0.88% +/- 0.06 for insulin-dependent diabetic patients. The low percentages of glycated C3 in both our in vitro and in vivo studies show that this pr otein is subject to only moderate rates of glycation.