Microsatellite markers have been established for a large number of spe
cies, but up till now very few polymorphic microsatellite markers have
been reported in poultry. We have isolated 34 polymorphic chicken mic
rosatellite markers of the poly (TG) type. The number of repeats varie
d from 9 up to 33. Often, other repeats such as poly (T) or poly (GAA)
were present adjacent to the poly (TG) repeat. Polymerase chain react
ion amplification of the microsatellites resulted in detection of thre
e or more alleles in a test panel of five different animals for 75% of
the microsatellites. Segregation of five microsatellite markers has b
een tested in a small family.