DR-78, A NOVEL DROSOPHILA-MELANOGASTER GENOMIC DNA FRAGMENT HIGHLY HOMOLOGOUS TO THE DNA-BINDING DOMAIN OF THYROID-HORMONE RETINOIC ACID VITAMIN-D RECEPTOR SUBFAMILY

Degenerate oligodeoxyribonucleotides were designed for both ends of th e DNA-binding domain of members of the nuclear receptor superfamily. P CR amplified Drosophila melanogaster DNA was purified and cloned (DR p lasmids). Genomic lambdaDASH clones were identified at high stringency with an amplified DR-78 plasmid DNA and isolated. The partial sequenc e shows a very probable open reading frame which would encode a peptid e highly homologous to members of the thyroid hormone-retinoic acid-vi tamin D receptor subfamily. The fragment corresponds to a single copy gene and was mapped at position 78D of chromosome three by in situ hyb ridization.