Tc. Hodgman et al., CHARACTERIZATION OF A BACILLUS-THURINGIENSIS STRAIN WHICH IS TOXIC TOTHE HOUSEFLY MUSCA-DOMESTICA, FEMS microbiology letters, 114(1), 1993, pp. 17-22
A Bacillus thuringiensis isolate has been discovered which is toxic to
the common housefly (Musca domestica) as well as other Diptera and Le
pidoptera. Crystal delta-endotoxins purified from this isolate killed
50% of Musca larvae at a concentration of 10.2 mu g/ml, and beta-exoto
xin was not detected. Sodium dodecyl polyacrylamide gel electrophoresi
s of the purified crystals revealed three protein species which were r
elated to CryIA(b), CryIB and CryIIA toxins on the basis of immunoreac
tivity and amino-terminal sequence determination. Southern blot and DN
A restriction analyses suggested that the strain has sequences related
to one cyvIA(b), one cryIIA, and two cryIIB genes.