ENZYME-CATALYZED SYNTHESIS OF RADIOLABELED 5-PREGNEN-3-BETA-OL-20-ONESULFATE

The enzymatic synthesis of [H-3]5-pregnen-3beta-ol-20-one sulfate usin g [H-3]5-pregnen-3beta-ol-20-one, 3'-phosphoadenosine-5'-phosphosulfat e and hydroxysteroid sulfotransferase 1 purified from rat liver is rep orted. The described procedure allowed the obtainment of high specific activity [H-3]5-pregnen-3beta-ol-20-one sulfate in yields ranging fro m 78 to 86% with respect to [H-3]5-pregnen-3beta-ol-20-one. Two-dimens ional thin-layer chromatography was used to purify [H-3]5-pregnen-3bet a-ol-20-one sulfate which upon solvolysis resulted in the formation of [H-3]5-pregnen-3beta-ol-20-one. The identity both of the synthesized compound and the solvolysed one was confirmed by reversed-phase high p ressure liquid chromatography, and 2-dimensional thin-layer chromatogr aphy.