CHARACTERIZATION OF THE OLIGOSACCHARIDE MOIETY OF VIP RECEPTOR FROM THE HUMAN PANCREATIC-CELL LINE BXPC-3

Authors
FABRE C ELBATTARI A BELLAN C PASQUALINI E MARVALDI J LOMBARDO D LUIS J
Citation
C. Fabre et al., CHARACTERIZATION OF THE OLIGOSACCHARIDE MOIETY OF VIP RECEPTOR FROM THE HUMAN PANCREATIC-CELL LINE BXPC-3, Peptides, 14(6), 1993, pp. 1331-1338
Citations number
39
Categorie Soggetti
Biology
Journal title
Peptides → ACNP
ISSN journal
01969781
Volume
14
Issue
6
Year of publication
1993
Pages
1331 - 1338
Database
ISI
SICI code
0196-9781(1993)14:6<1331:COTOMO>2.0.ZU;2-R
Abstract
The human pancreatic cell line BxPC-3 displays two classes of binding sites with high and low affinity for VIP. The order of potency of VIP- related peptides in inhibiting either [I-125]VIP or [I-125]N-AcPACAP27 binding and in stimulating cAMP production was typical of the human V IP receptor. By combining affinity labeling with glycosidase treatment s, we have characterized the VIP receptor as a M(r) = 68,200 glycoprot ein. consisting of a M(r) = 39,300 polypeptide core with at least thre e N-linked oligosaccharide chains. In addition, our results revealed t he presence of a low amount of sialic acid residues in the carbohydrat e moiety of receptor.