ENHANCED REPLICATION OF PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME(PRRS) VIRUS IN A HOMOGENEOUS SUBPOPULATION OF MA-104 CELL-LINE

Authors
KIM HS KWANG J YOON IJ JOO HS FREY ML
Citation
Hs. Kim et al., ENHANCED REPLICATION OF PORCINE REPRODUCTIVE AND RESPIRATORY SYNDROME(PRRS) VIRUS IN A HOMOGENEOUS SUBPOPULATION OF MA-104 CELL-LINE, Archives of virology, 133(3-4), 1993, pp. 477-483
Citations number
14
Categorie Soggetti
Virology
Journal title
Archives of virology → ACNP
ISSN journal
03048608
Volume
133
Issue
3-4
Year of publication
1993
Pages
477 - 483
Database
ISI
SICI code
0304-8608(1993)133:3-4<477:EROPRA>2.0.ZU;2-1
Abstract
Two different cell populations, high- (MARC-145) and low-permissive ce ll clones (L-1) to porcine reproductive and respiratory syndrome (PRRS ) virus, were derived from MA-104 cell line (parent cell: P) by cell c loning. Maximum virus yields in MARC-145, P, and L-1 cell clones were 10(8.5), 10(3.5), and 10(2.5) tissue culture infective dose 50 (TCID50 )/0.1 ml, respectively. The MARC-145 cell clone supported replication of all 11 different porcine reproductive and respiratory syndrome viru s isolates that were tested. These results indicated that the MARC-145 cells will be useful for PRRS virus replication.