THE FOCUSING POSITIONS OF POLYPEPTIDES IN IMMOBILIZED PH GRADIENTS CAN BE PREDICTED FROM THEIR AMINO-ACID-SEQUENCES

Authors
BJELLQVIST B HUGHES GJ PASQUALI C PAQUET N RAVIER F SANCHEZ JC FRUTIGER S HOCHSTRASSER D
Citation
B. Bjellqvist et al., THE FOCUSING POSITIONS OF POLYPEPTIDES IN IMMOBILIZED PH GRADIENTS CAN BE PREDICTED FROM THEIR AMINO-ACID-SEQUENCES, Electrophoresis, 14(10), 1993, pp. 1023-1031
Citations number
27
Categorie Soggetti
Biochemical Research Methods
Journal title
Electrophoresis → ACNP
ISSN journal
01730835
Volume
14
Issue
10
Year of publication
1993
Pages
1023 - 1031
Database
ISI
SICI code
0173-0835(1993)14:10<1023:TFPOPI>2.0.ZU;2-2
Abstract
The focusing Positions in narrow range immobilized pH gradients of 29 polypeptides of known amino acid sequence were determined under denatu ring conditions. The isoelectric points of the proteins calculated fro m their amino acid sequences matched with good accuracy the experiment ally determined pI values. We show the advantages of being able to pre dict the position of a protein of known structure within a two-dimensi onal gel.