DETECTION OF 3 PLANT-VIRUSES BY DOT-BLOT AND TISSUE-BLOT IMMUNOASSAYSUSING CHEMILUMINESCENT AND CHROMOGENIC SUBSTRATES

Detection of bean yellow mosaic virus (BYMV), impatiens necrotic spot virus (INSV) and lily symptomless virus (LSV) by dot-blot and tissue-b lot immunoenzymatic assay was compared by using either chromogenic or chemiluminescent substrates. Testing was conducted on infected-leaf ex tracts and on purified virus preparations. Both types of substrates ga ve comparable results with leaf extracts. However, with purified virus , the use of chemiluminescent substrates led to a more sensitive detec tion of the three viruses.