Db. Volkin et al., SUCRALFATE AND SOLUBLE SUCROSE OCTASULFATE BIND AND STABILIZE ACIDIC FIBROBLAST GROWTH-FACTOR, Biochimica et biophysica acta, 1203(1), 1993, pp. 18-26
The actions of the anti-ulcer drug sucralfate have been proposed to be
mediated through interaction with fibroblast growth factors (Folkman,
J., Szabo, S., Strovroff, M., McNeil, P., Li, W. and Shing, Y. (1991)
Ann. Surg. 214, 414-427). We show here that acidic fibroblast growth
factor (aFGF; FGF-1) binds in vitro to both the soluble potassium salt
and the insoluble aluminum salt of sucrose octasulfate, as demonstrat
ed by a variety of biophysical techniques. Similar to the well-describ
ed interaction and stabilization of aFGF by heparin, soluble sucrose o
ctasulfate (SOS) stabilizes aFGF against thermal, urea and acidic pH-i
nduced unfolding as determined by a combination of circular dichroism,
fluorescence spectroscopy and differential scanning calorimetry. In a
ddition, SOS also enhances the mitogenic activity of aFGF and partiall
y protects the protein's three cysteine residues from copper-catalyzed
oxidation. SOS competes with heparin and suramin for the aFGF polyani
on binding site as measured by both fluorescence and light scattering
based competitive binding assays. Front-face fluorescence measurements
show that the native, folded form of aFGF binds to the insoluble alum
inum salt of sucrose octasulfate (sucralfate). Moreover, sucralfate st
abilizes aFGF against thermal and acidic pH-induced unfolding to the s
ame extent as observed with SOS. Thus, due to their high charge densit
y, SOS and sucralfate bind and stabilize aFGF via interaction with the
aFGF polyanion binding site.