DETECTION OF ALDICARB SULFONE AND CARBOFURAN IN FORTIFIED MEAT AND LIVER WITH COMMERCIAL ELISA KITS AFTER RAPID EXTRACTION

Rapid methods of extraction were developed to screen for the presence of carbofuran and aldicarb sulfone in meat and liver using commercial ELISA kits for analysis. The general extraction procedure consisted of blending the ground meat or liver with water or acetonitrile, filteri ng or centrifuging the extract, and then performing ELISA with commerc ially available kits. This procedure helped determine the insecticides fortified at concentrations near or below the regulatory tolerance le vels for carbofuran and aldicarb in these matrices. Assays for aldicar b sulfone were also carried out directly in bovine milk, blood, and ur ine. The extraction procedure was also applied to the Enzytec biosenso r ticket that is based on cholinesterase inhibition.