CHANGES IN ESTERASE ISOENZYME PATTERNS IN GRAINS OF SPRING BARLEY (HORDEUM-VULGARE L) AFTER THE ATTACK BY GRAIN WEEVIL (CALANDRA-GRANARIA L) AND THE GRAIN WEEVIL PREFERENCE FOR INDIVIDUAL CULTIVARS

We have found relationship between the impact of Calandra granaria L. in barley grains and the changes of esterase isoenzyme patterns. The d estruction of endospermal tissues, sometimes at the starting period ha rdly macroscopically visible, is accompanied by the thinning or disapp earance of the respective tissue specific esterase isoenzymes (Rm 21 a nd 28). By a stronger impact all the esterase isoenzymes are gradually reduced or disappear, including most intensive ones. Hordein fraction s, on the other hand, remain intact as in the control. In some cases ( especially in cv. Bonus) a diffuse additional X zone appears at Rm 74- 77. Its origin is not clear yet, but in the analysis of the grain weev il appears on esterase zymogram a band in the same position as this on e This zone was never present in the intact grains, hence its appearan ce can reliably signalize the attack by Calandra granaria even in grai ns, where its attack is not visible macroscopically. We have also exam ined the grain weevil preference for individual cultivars. The highest damage was found in grains of cv. Koral, Novum, Krystal, me medium da mage was in cv. Orbit and Jaspis, grains of cv. Mars, Malvaz, Fatran a nd Jarek were damaged very little. This has been confirmed both macros copically and by analysis of esterase isoenzyme patterns.