DETERMINATION OF PACLITAXEL IN BIOLOGICAL-FLUIDS BY MICELLAR ELECTROKINETIC CHROMATOGRAPHY

Authors
HEMPEL G LEHMKUHL D KRUMPELMANN S BLASCHKE G BOOS J
Citation
G. Hempel et al., DETERMINATION OF PACLITAXEL IN BIOLOGICAL-FLUIDS BY MICELLAR ELECTROKINETIC CHROMATOGRAPHY, Journal of chromatography, 745(1-2), 1996, pp. 173-179
Citations number
13
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography → ACNP
Volume
745
Issue
1-2
Year of publication
1996
Pages
173 - 179
Database
ISI
SICI code
Abstract
A method has been developed for the determination of paclitaxel (Taxol ) in plasma and urine using capillary electrophoresis with sodium dode cyl sulfate as additive in the run buffer. The samples are extracted a nd preconcentrated with tert.-butyl methyl ether. Taxotere has been us ed as the internal standard. The limit of detection for paclitaxel is 20 ng/ml. In comparison to high-performance liquid chromatography, the capillary electrophoresis method is simple and needs less organic sol vents.