DEPOLARIZATION OF RAMAN-SCATTERING FROM SOME NUCLEOTIDES OF RNA AND DNA

Depolarization ratios of Raman bands, excited at 488.0 nm, of guanosin e-5'-monophosphoric acid, cytidine-5'-monophosphoric acid, adenosine-5 '-monophosphoric acid, thymidine-5'-monophosphoric acid, and uridine-5 '-monophosphoric acid have been measured in their H2O and D2O solution s in the spectral region from 300 to 1800 cm(-1). For comparison, the disodium salt of 2'-deoxyadenosine-5'-monophosphoric acid was also sub jected to the depolarization measurement in its H2O solution. The resu lts have been correlated with possible orientations of the principal a xes of the Raman scattering tensors as well as with the relative magni tudes of the tenser components. Results should be useful for future po larized Raman studies of synthetic and natural DNA. (C) 1993 John Wile y and Sons, Inc.