H. Yabushita et Ac. Sartorelli, EFFECTS OF SODIUM-BUTYRATE, DIMETHYLSULFOXIDE AND DIBUTYRYL-CAMP ON THE POORLY DIFFERENTIATED OVARIAN ADENOCARCINOMA CELL-LINE AMOC-2, Oncology research, 5(4-5), 1993, pp. 173-182
To determine whether the poorly differentiated AMOC-2 human ovarian ad
enocarcinoma cell line was capable of undergoing differentiation, AMOC
-2 cells were exposed to 2 mM sodium butyrate, 2.5% dimethylsulfoxide,
or 4 mM dibutyryl cyclic adenosine monophosphate (dibutyryl cAMP) for
6 days. These treatments resulted in growth inhibition, a reduction i
n clonogenicity and an increase in cellular glycogen content. Signific
ant increases in heat stable alkaline phosphatase activity also occurr
ed after exposure to sodium butyrate. In addition, a thorn-like microf
ilament structure observed in untreated cells was diminished concomita
ntly with morphological changes that included flattening, enlargement
and extended cytoplasmic processes after exposure to sodium butyrate o
r dibutyryl cAMP. Furthermore, treatment with sodium butyrate increase
d the intracellular concentrations of beta-tubulin, vimentin, neurofil
aments (M(r) 210,000) and cytokeratin (M(r) 56,000-58,000). These chan
ges were completely reversed after removal of the inducing agent. The
findings suggest that treatment of AMOC-2 cells with sodium butyrate i
nduced a more differentiated phenotype, although terminal differentiat
ion was not achieved.