COSMID MAPPING AND LOCUS LINKAGE WITHIN THE HUMAN CHROMOSOMAL REGION 22Q13.1

Many human meningiomas show loss of heterozygosity at distal loci but retain constitutional heterozygosity at one or more proximal loci of 2 2q. Molecular analysis indicted deletions involving at least the regio n 22q12.3qter. In this region, distal to myoglobin, the putative menin gioma locus ought to be expected. Long-range mapping was performed aro und two loci from 22q12.3-q13.1 (D22S16 and PDGFB, the most proximal l ocus to be lost in meningioma). D22S16, originally assigned to 22q13-q ter by isotopic in situ hybridization, was placed in the vicinity of P DGFB by utilizing a set of somatic cell hybrids, an assignment confirm ed by fluorescence in situ hybridization (FISH) of a cosmid clone cont aining the D22S16 locus. Moreover, pulsed field gel electrophoresis su ggests a close linkage of both markers within 630 kb.