PRODUCTION OF MONOCLONAL-ANTIBODIES RECOGNIZING DIFFERENT EPITOPES PRESENT ON INSULIN-LIKE GROWTH FACTOR-I

Monoclonal antibodies (MAbs) were generated against recombinant human insulin-like growth factor 1 (IGF-1) by fusion of NS-1 myeloma cells w ith spleen cells from BALB/C X DBA mice immunised with recombinant IGF -1 and synthetic peptide sequences derived from the published amino ac id sequence of IGF-1. MAbs were produced that recognised four distinct epitopes including two defined segments of the C and D domains. All M Abs were IgM mouse immunoglobulins. These results indicate the feasibi lity of producing MAbs to highly conserved proteins.