ENDOTOXIN-INDUCED ARTERIAL ENDOTHELIAL BARRIER DYSFUNCTION ASSESSED BY AN IN-VITRO MODEL

Using an in vitro model in which albumin transfer across monolayers of bovine aortic endothelial cells (BAEC) was measured, we have shown th at lipopolysaccharide (LPS) induces a concentration-dependent increase in endothelial permeability. This increase was biphasic, having an ea rly peak at 2 h and rising again by 24 h. Both peaks were abolished by polymixin B (PMB) but were unaffected by N(omega)-monomethyl-L-argini ne, N(omega)-nitro-L-arginine methyl ester or dexamethasone. Furthermo re, LPS did not stimulate nitric oxide production by BAEC following 24 h exposure. Thus, the LPS-induced increase in permeability may accoun t for the vascular leakage of septic shock, but the L-arginine-nitric oxide system does not appear to be involved.