EFFECT OF METAL-IONS ON THE FORMATION AND DESTRUCTION OF HYDROGEN-PEROXIDE IN MEDIA USED FOR MAMMALIAN ADIPOCYTES INCUBATION

The activity of transition metals under incubation conditions is of pa rticular interest because these metal ions induced an insulin-like eff ect in fat cells which is reminiscent of that of hydrogen peroxide. It is possible that in one of its bound states nickel may stimulate hydr ogen or lipid peroxide accumulation which in turn can account for this effect on adipocytes during in vitro incubation. In this study using a simple model system the role of metal ions on the formation and dest ruction of hydrogen peroxide is illustrated in medium used for mammali an adipocytes in-cubation. It is interesting to note that the only met al which generated hydrogen peroxide in the medium was copper when its concentration was 10 muM. The metals associated with destruction of h ydrogen peroxide were cobalt, copper and manganese. Iron and nickel pl ayed no role at all.