DETECTION OF ESTABLISHED AND NOVEL ALLELES OF THE HLA-DPB1 LOCUS BY PCR-SSO

Authors
PICKL WF FAE I FISCHER GF
Citation
Wf. Pickl et al., DETECTION OF ESTABLISHED AND NOVEL ALLELES OF THE HLA-DPB1 LOCUS BY PCR-SSO, Vox sanguinis, 65(4), 1993, pp. 316-319
Citations number
20
Categorie Soggetti
Hematology
Journal title
Vox sanguinis → ACNP
ISSN journal
00429007
Volume
65
Issue
4
Year of publication
1993
Pages
316 - 319
Database
ISI
SICI code
0042-9007(1993)65:4<316:DOEANA>2.0.ZU;2-D
Abstract
The polymorphism at the HLA-DPB1 locus was investigated in 490 Austria n individuals. Exon 2 sequences of the HLA-DPB1 genes were amplified b y polymerase chain reaction and probed with a panel of sequence-specif ic oligonucleotide probes. HLA-DPB1 genotypes were deduced from the hy bridization pattern of the probes. HLA-DPB1 allele frequencies and Har dy-Weinberg equilibrium were calculated. Linkage disequilibria between HLA-DPB1 and HLA-DQA1 alleles were determined. Four novel hybridizati on patterns indicating new alleles were found.