D. Acunacastroviejo et Mi. Pablos, MELATONIN RECEPTORS IN PURIFIED CELL-NUCLEI OF LIVER, Research communications in chemical pathology and pharmacology, 82(2), 1993, pp. 253-256
Specific binding of [I-125]iodomelatonin to homogenates from purified
rat liver nuclei was characterized. The binding is rapid, reversible.
saturable and of high affinity. Specific binding seems to be found in
the nuclear protein fraction, since after precipitation of the protein
s with trichloroacetic acid, the specific binding disappeared. The K(d
) (180 +/- 20 pM) and B(max) (9.1 +/- 0.03 fmol/mg protein) values (me
an +/- S.E.M.) agree with the melatonin concentration in nuclei and ma
y imply a physiological locus for melatonin action.