MELATONIN RECEPTORS IN PURIFIED CELL-NUCLEI OF LIVER

Authors
ACUNACASTROVIEJO D PABLOS MI
Citation
D. Acunacastroviejo et Mi. Pablos, MELATONIN RECEPTORS IN PURIFIED CELL-NUCLEI OF LIVER, Research communications in chemical pathology and pharmacology, 82(2), 1993, pp. 253-256
Citations number
10
Categorie Soggetti
Pharmacology & Pharmacy",Pathology
Journal title
Research communications in chemical pathology and pharmacology → ACNP
ISSN journal
00345164
Volume
82
Issue
2
Year of publication
1993
Pages
253 - 256
Database
ISI
SICI code
0034-5164(1993)82:2<253:MRIPCO>2.0.ZU;2-8
Abstract
Specific binding of [I-125]iodomelatonin to homogenates from purified rat liver nuclei was characterized. The binding is rapid, reversible. saturable and of high affinity. Specific binding seems to be found in the nuclear protein fraction, since after precipitation of the protein s with trichloroacetic acid, the specific binding disappeared. The K(d ) (180 +/- 20 pM) and B(max) (9.1 +/- 0.03 fmol/mg protein) values (me an +/- S.E.M.) agree with the melatonin concentration in nuclei and ma y imply a physiological locus for melatonin action.