ACYCLIC ANALOGS OF 2',3'-DIDEOXY-2',3'-DIDEHYDRONUCLEOSIDE 5'-TRIPHOSPHATES, TERMINATION SUBSTRATES IN DNA-SYNTHESIS BY A RANGE OF DNA-POLYMERASES

Authors
VIKTOROVA LS MOZZHERIN DY ROZOVSKAYA TA KUCHANOVA MK KRAYEVSKY AA
Citation
Ls. Viktorova et al., ACYCLIC ANALOGS OF 2',3'-DIDEOXY-2',3'-DIDEHYDRONUCLEOSIDE 5'-TRIPHOSPHATES, TERMINATION SUBSTRATES IN DNA-SYNTHESIS BY A RANGE OF DNA-POLYMERASES, Molecular biology, 27(1), 1993, pp. 85-90
Citations number
21
Categorie Soggetti
Biology
Journal title
Molecular biology → ACNP
ISSN journal
00268933
Volume
27
Issue
1
Year of publication
1993
Part
2
Pages
85 - 90
Database
ISI
SICI code
0026-8933(1993)27:1<85:AAO25>2.0.ZU;2-8
Abstract
O-4'-nor-2',3'-dideoxy-2',3'-didehydronucleoside 5'-triphosphates (acy clo-d4NTP) are shown to be efficient termination substrates in DNA bio synthesis catalyzed by human placental DNA polymerases alpha and epsil on, rat liver DNA polymerase beta, reverse transcriptases of human imm unodeficiency virus and avian myeloblastosis virus, and calf thymus te rminal deoxynucleotidyl transferase. Among the enzymes studied, these compounds do not interact only with the Escherichia coli DNA polymeras e I (Klenow fragment). The possible causes of acyclo-d4NTP interaction with DNA-synthesizing complexes are discussed.