HUMAN PLACENTA 40S RIBOSOMAL-SUBUNIT AFFINITY MODIFICATION BY OLIGORIBONUCLEOTIDE DERIVATIVES CONTAINING THE AUG CODON - RIBOSOMAL-PROTEINSCOMPOSING THE CODON ANTICONDON INTERACTION SITE

Human placenta 40S ribosomal initiation complexes were obtained using 40S ribosomal subunits and eIF-2 . GTP . Met-tRNA(f)Met and then modif ied with O-[4-N-(2-chloroethyl)-N-N-methylamino]benzylidene derivative s of AUGU(n)[P-32]pC and hloroethyl)-N-methylamino]benzylmethylphospho amide derivatives of [P-32] pAUGU(n) oligoribonucleotides. After the i nteraction with these mRNA analogs, the modified ribosomal proteins we re analyzed. It was shown that in the presence of the ternary eIF-2 . GTP . Met-tRNA(f)Met complex, the S3/S3a, S6, S7, and S14/S15 proteins play the key role in the 40s subunit-template interactions; the S2, S 4, S5, S8, S9, and S17 proteins are also involved.