ENZYME-MEDIATED SYNTHESIS OF 2 DIASTEREOISOMERIC FORMS OF PHOSPHATIDYLGLYCEROL AND OF DIPHOSPHATIDYLGLYCEROL (CARDIOLIPIN)

3-sn-Phosphatidyl-1'-sn-glycerol and its stereoisomer 3-sn-phosphatidy l-3'-sn-glycerol are prepared through phospholipase D-catalysed transp hosphatidylation of natural phosphatidylcholine with the two enantiome ric (R)- and (S)-isopropylideneglycerols and subsequent hydrolysis of the phospholipids obtained. If glycerol is the alcohol donor in the sa me reaction, a mixture of stereoisomers is obtained. When the enzyme f rom savoy cabbage is used, the two compounds are obtained as the only products. With PLD from Streptomyces as catalyst, the initially formed phosphatidylglycerols are quantitatively transformed into diphosphati dylglycerol (cardiolipin).