Dj. Harrison et al., RAPID SEPARATION OF FLUORESCEIN DERIVATIVES USING A MICROMACHINED CAPILLARY ELECTROPHORESIS SYSTEM, Analytica chimica acta, 283(1), 1993, pp. 361-366
Using micromachining techniques electrophoresis systems consisting of
sample injectors and separation capillaries have been fabricated in pl
anar glass structures. A device with a total capillary channel length
of 13.9 cm was used to inject and separate fluorescein and fluorescein
sulfonate dyes within 3 min, with an injector to detector distance of
6.0 cin, and an applied field of 520 V/cm. A similar device with a ca
pillary length of 1.6 cm effected injection and separation within 4 s
for an injector to detector length of 0.75 cm at an applied field of 1
875 V/cm. Injected sample volumes of 60 pl or less were readily inject
ed and analyte detected at 10 muM concentrations.