ANALYSIS OF LIGNAN GLYCOSIDES IN SESAME S EED BY HIGH-PRESSURE LIQUID-CHROMATOGRAPHY

Recently, it was suggested that lignan glycosides would exist in an oi l cake of sesame seed. However, until now, the amount or composition o f lignan glycosides were not reported exactly. We analyzed ligan glyco sides in sesame seed by high-pressure liquid chromatography. A mixture of glycosides was extracted with aqueous methanol from the oil cake. The aglycons of each glycoside were identified by GC-MS and the number of sugar residues was estimated by LC-MS. Eight ligan glycosides were found, and they were eluted during reversed-phase HPLC in the expecte d order. Lignan glycosides could be hydrolyzed with a combination of c ellulase and beta-glucosidase.