EUKARYOTIC ACTIVATORS FUNCTION DURING MULTIPLE STEPS OF PREINITIATIONCOMPLEX ASSEMBLY

Authors
Citation
B. Choy et Mr. Green, EUKARYOTIC ACTIVATORS FUNCTION DURING MULTIPLE STEPS OF PREINITIATIONCOMPLEX ASSEMBLY, Nature, 366(6455), 1993, pp. 531-536
Citations number
50
Categorie Soggetti
Multidisciplinary Sciences
Journal title
NatureACNP
ISSN journal
00280836
Volume
366
Issue
6455
Year of publication
1993
Pages
531 - 536
Database
ISI
SICI code
0028-0836(1993)366:6455<531:EAFDMS>2.0.ZU;2-7
Abstract
Eukaryotic activator proteins (activators) stimulate transcription by increasing assembly of the preinitiation complex. We have developed me thods to quantify the stable assembly of general transcription factors into transcriptional complexes in response to activators. We show tha t activators function during at least two stages of preinitiation comp lex assembly: first, to recruit the general transcription factor TFIIB , and then at a second step, after TFIIB entry. It is at this second s tep that the TATA-box binding protein associated factors act. This ste p also seems to be critical for activators to stimulate transcription synergistically.